Plant Transformation

Location

Morgenstelle 1
2. Stock, Raum Nr.218

Morgenstelle 32
5. Stock, Raum 5X17

phone 73223


Booking Trafos

Equipment

4 sterile benches, growth rooms

sterile bench

 

Our New Bench: Mission Impossible...


Coordinator

Dr. Kenneth W Berendzen


Transformation technicians

Caterina Brancato
Caterina Brancato

 

 



 

Nach oben



Service provided by Anja and Caterina

Transformation Unit Protocols

Please cite: Wittmann et al. 2015. Plant Pathology. Doi: 10.1111/ppa.12417
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12417/abstract

1. The unit offers the service of Agrobacterium-mediated transformation of potato, tobacco and tomato.
2. Regenaration and cultivation of transformants.
3. PEG-mediated transformations of protoplasts from cell cultures or plants-mesophyl (Arabidopsis or tobacco).
4. Productions of cell cultures (wild type).





Facs

Calculation of the protoplat transformation efficiency by flow cytometric anaylsis (FCA).

 

Protoplasts isolated from root cell culture are transformed with a 35S::GFP construct (12kb) as a routine control. As an alternative to calculating the transformation efficiency, we have used FCA as a means for detecting cells expressing GFP. The negative population is marked in black. Cells that are strongly expressing GFP have a fluoresence signal that is well above that of the total plant cell population‘s green autofluoresence (cells marked in dark green); cells marked in light green are those with weaker GFP fluoresence emission. In this example 23% of the cells were transformed with GFP, and 50% of those are strongly expressing GFP.



 

 

Anja Hoffmann








Publications


Albert I, Böhm H, Albert M, Feiler CE, Imkampe J, Wallmeroth N, Brancato C, Raaymakers TM, Oome S, Zhang H, Krol E, Grefen C, Gust A, Chai J, Hedrich R, Van den Ackerveken G, Nürnberger T (2015). An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity. Nature Plants, 15140, doi: 10.1038/NPLANTS.2015.140.

J. Wittmann, C. Brancato, K. W. Berendzen and B. Dreiseikelmann (2015).
Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene.
Plant Pathology
DOI: 10.1111/ppa.12417

 

 

ZMBP Home

Contact

Dr. Kenneth Berendzen


ZMBP - Universität Tübingen
Auf der Morgenstelle 32
72076 Tübingen
phone 73223
email kenneth.berendzen[at]zmbp.uni-tuebingen.de




Caterina Brancato

ZMBP - Universität Tübingen
Auf der Morgenstelle 32
72076 Tübingen
phone 73223
email caterina.brancato[at]zmbp.uni-tuebingen.de